Fluorescence in situ hybridization on vibratome sections of plant tissues
نویسندگان
چکیده
منابع مشابه
Fluorescence in Situ Hybridization (fish) on Vibratome Sections of Plant Tissues
This protocol describes the application of fluorescence in situ hybridization (FISH) on three-dimensionally preserved tissue sections derived from intact plant structures such as roots or florets. The method is based on the combination of vibratome sectioning with confocal microscopy. The protocol provides an excellent tool to investigate chromosome organization in plant nuclei in all cell type...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2007
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2007.265